LIFE MagMAX™ Cell-Free DNA Isolation Kit現貨
LIFE MagMAX™ Cell-Free DNA Isolation Kit現貨
MagMAX™ 磁珠凈化技術的優點:
在從細胞中分離RNA和從無細胞樣本中分離RNA方面，磁珠具有很多優點。Ambion的 MagMAX技術比玻璃纖維濾膜法的RNA結合效率更高，所以提高了RNA產量。不論是實驗與實驗之間，還是多種長度不同的樣本之間，RNA產量的穩定性都更高。MagMAX磁珠分離技術避免了樣本中細胞微粒引起的濾膜堵塞問題。因為進行高效的連接反應只需使用少量的磁珠，所以僅用 20–50 μL 去核酸酶水就可洗脫連接的RNA，大量稀釋樣本中濃縮RNA。
優化用于從生物液體中分離病毒:
Ambion® MagMAX™ 病毒DNA分離試劑盒已經過優化，與生物體液以及血清、血漿、拭子和細胞培養基等無細胞樣本配套使用。相對于從組織或培養細胞中分離RNA而言，從無細胞樣本中分離RNA有其*的困難。這些困難包括病毒滴度低、待處理樣本量大和樣本復雜性。另外，操作程序必須與實時熒光定量逆轉錄PCR相兼容。 MagMAX™ 病毒DNA分離試劑盒可以解決所有這些困難，無需使用有機溶劑和進行RNA沉淀，可避免堵塞、洗脫量大和產量不穩定等基于濾膜的RNA分離方法中經常遇到的問題。從1/10的少量樣品中獲得等量的RNA病毒檢測靈敏度已經過 Ambion's MagMAX™ 實驗方案和現行國家獸醫服務實驗室（NVSL）驗證的病毒檢測實驗方案分離出來的RNA進行了比較。即使在樣品投入量為1/10時，用 MagMAX™ 試劑盒分離的病毒RNA水平還是等于或高于NVSL實驗方案以及推薦的同行試劑盒所分離出來的RNA（見數據）。在我們的自動化資源中可獲取可下載的實驗方案，用于利用特殊的液體處理系統使用該試劑盒。
LIFE MagMAX™ Cell-Free DNA Isolation Kit現貨
The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma. The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.
Scalable isolation of circulating nucleic acid from plasma, serum, and urine
cfDNA recovery is challenging due to the limited amount present in cell-free samples. The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 500 µL to 10 mL. When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.
Features of the MagMAX Cell-Free DNA Isolation Kit include:
• Flexible design that permits both manual and automated isolation of cfDNA
• Scalable format that allows for higher and lower input volumes
• Automation-ready, phenol-free extraction
• Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head
• Wide sample volume inputs range from 500 µL to 10 mL of plasma/serum or urine
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA
• Elution volumes range from 15 µL to 50 µL
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