qiagen質粒大量提取試劑盒12162 12163
qiagen質粒大量提取試劑盒12162 12163
QIAGEN Plasmid Kit procedures.
Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
qiagen質粒大量提取試劑盒12162 12163
Performance
QIAGEN Plasmid Kit利用重力流QIAGEN陰離子交換柱技術高效純化質粒DNA。產量可達10 mg（Giga）、2.5 mg（Mega）、500 µg（Maxi）、或100 µg（Midi）高拷貝質粒DNA（培養體積取決于質粒拷貝數、插入片段大小、宿主菌株和培養基。）QIAGEN Plasmid Kits純化的質粒DNA適合多種應用
訂購信息：
12162質粒大量提取試劑盒QIAGEN Plasmid Maxi Kit (10) 規格：10次
12163質粒大量提取試劑盒QIAGEN Plasmid Maxi Kit (25) 規格：25次
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